Histological implication of p53 gene expression in oral squamous cell carcinoma

 
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Karima Al Salihi1*, Ihsan Abdullah2, S. L. Ang3

Abstract

This study intends to detect the TP53 genetic alterations and mutations in the conserved regions of the TP53 gene in tumor tissue collected from oral squamous cell carcinoma patients (OSCC), using polymerase chain reaction (PCR) and direct sequencing, as well as to evaluate the expression of P53 by immunohistochemistry and its relation to histopathological changes. The study was conducted on 15 resected specimens of SCC lesions of the tongue, buccal mucosa and floor of the mouth. Slides from these specimens were evaluated for histological alterations and p53 over-expression by immunohistochemistry. Also, extracted DNA amplified using PCR and gene sequencing was done to detect the genetic mutation. The results of this study revealed a significant correlation between the total histological features and p53 over-expression. Ten out of 15(66.66%) patients were revealed P53 gene mutations, analyzed within exons 5- 8. The positive nuclear staining by immunohistochemical was observed in 13 cases (13/15, 86.66 %), moreover, 2 individual histological parameters that pointed out the high cellular turnover were also significantly associated with p53 over-expression. In conclusion, TP53 mutation histologically signifies the event in the cellular progression of oral SCC. Moreover, the result of this study indicate the high incidence of p53 protein over-expression, as well as specific mutations in oral SCC patients.

Keywords: Oral squamous cell carcinoma; PCR, TP53 mutation; WDSCC

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DOI: 10.18081/jcc/016-06/60-69

 
Research Article
Journal of Cellular Cancer
Volume 8, Issue 1, pages 60-69
Received February 22, 2016;
accepted May 21, 2016;
published June 29, 2016
 

How to cite this article

Al Salihi k, Abdullah I, Ang SL. Histological implication of p53 gene expression in oral squamous cell carcinoma. Journal of Cellular Cancer 2016;8(1)60-69.

Article outline

Abstract
Keywords
1. Introduction
2. Materials and methods
3. Results
4. Discussions
Acknowledgements
References

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