Detection of (HPV) using in situ hybridization molecular method in cervical lesions of Iraqi women

 
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Suhad Faisal Hatem Al mugdadi¹, Ban abbas abd- almajeed², Maha Mahamommed Alsayyid³, Zahraa Amer¹, Shahad Ayad¹

Abstract

Human papilloma virus is a group of genetically related viruses that commonly infect stratified squamous epithelium. HPVs induce proliferative changes that result in both benign and malignant tumors. HPV causes a mixture of lesions ranging from common warts to cervical neoplasia and cancer. In Situ hybridization ISH considered a viable potential option to molecular assays in the cervical lesions is depend on the Complementary pairing of labeled DNA or RNA  Probes beside  normal or abnormal nucleic acid Sequences in intact chromosomes, cells or tissue sections. This study covered the period from July&August 2015 involved the in situ hyperdization (ISH ) technique. Randomly, thirty four women patients were recruited at the  oncology teaching hospital medical city teaching center/Baghdad. samples were next submit to In situ hybridization (ISH) procedure for HPV. In situ hybridization detection system from Dako/UK was used to detect HPV 16&18 according to the manufactures instructions. Current results found the positive HPV in cervical lesions represented (79.4%) with significant differences (**P<0.01), while the negative HPV cervical lesions represented (20.6%). Age group (34-44) was the  highest frequency and percentage 17 (63%) of patients positive cervical lesions. the histopathology and cytology diagnosis showed that a high percentage in the patients under our study have positive HPV in cervical lesions represented (79.4%).In addition positive nuclear cells expression of HPV in two type of samples (cytology and Histopathology) were examined, histopathological samples ( 11/15, 73.3% ) and cytology samples (12/15,80%)  were positive to HPV in cervical lesions . while 4/4, 100% was positive HPV for both sample types. Expression pattern was highly significant as punctated and diffused (48.2%) compared to Punctate expression(7.4%). In conclusion, HPV detection in cytological specimens and histological by ISH molecular method were help to distinguish integrated HPV- DNA form, thus allowing conclusions about the prognosis of the cervical lesions abnormalities.

Keywords: Human papilloma virus; Cervix; In situ hybridization

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DOI: 10.18081/jcc/016-06/52-59

 
Research Article
Journal of Cellular Cancer
Volume 8, Issue 1, pages 52-59
Received March 03, 2016;
accepted June 07, 2016;
published June 29, 2016
 

How to cite this articleAl mugdadi S, Abd- almajeed B, Alsayyid M, Amer Z, Ayad S. Detection of (HPV) using  in situ  hybridization molecular method in cervical lesions of  Iraqi women. Journal of Cellular Cancer 2016;8(1)52-59.

Article outline

Abstract
Keywords
1. Introduction
2. Materials and methods
3. Results
4. Discussions
Acknowledgements
References

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