Real time PCR detection chromosomal translocation in lymphoma by special probe

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http://dx.doi.org/10.18081/jcc/016-06/28-33
Journal of Cellular Cancer  Volume 8, Issue 1, pages 28-33
Received January 21, 2016; accepted May 28, 2016; published June 28, 2016

Thana Mohammed Juda1*, Seenaa Badr1

Abstract

The research of malignancy was mostly reach to facts that the cancer is multifactorial, many risk factors are associated with developing of cancer in normal cell so we always need for a new and accurate methods in early diagnosis and identify persons susceptible for developing cancer and new technique need in follow up. One of important risk factor which are associated with developing cancer are mutation, in this work we study the association between the t(14;18) (q32;q21) translocation between BCL2 gene on chromosome 14 and immunoglobulin heavy chain on chromosome 18. Detection of this translocation can facilitate the diagnosis of follicular lymphoma and can be used to monitor response to therapy and level of residual disease. We here collect sample of non-Hodgkin lymphoma from lab in Babylon city  and we depend on paraffin embedded tissue as diagnosed by histopathologists, we extract DNA and prepare special primer for amplification the translocation and we depend on technique of real time PCR as diagnostic procedure for translocation detection by special probe (fam-tamra probe) which give positive signal in non-Hodgkin lymphoma and Burkett’s lymphoma by compares with control which are tonsil tissue which give negative signal which indicate that translocation can be considered as risk factor for developing lymphoma.

Keywords: Lymphoma; PCR; DNA; Multi factorial; Non-Hodgkin

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