Suhad Faisal Hatem Al mugdadi¹, Ban abbas abd- almajeed², Maha Mahamommed Alsayyid³, Zahraa Amer¹, Shahad Ayad¹
Abstract
Human papilloma virus is a group of genetically related viruses that commonly infect stratified squamous epithelium. HPVs induce proliferative changes that result in both benign and malignant tumors. HPV causes a mixture of lesions ranging from common warts to cervical neoplasia and cancer. In Situ hybridization ISH considered a viable potential option to molecular assays in the cervical lesions is depend on the Complementary pairing of labeled DNA or RNA Probes beside normal or abnormal nucleic acid Sequences in intact chromosomes, cells or tissue sections. This study covered the period from July&August 2015 involved the in situ hyperdization (ISH ) technique. Randomly, thirty four women patients were recruited at the oncology teaching hospital medical city teaching center/Baghdad. samples were next submit to In situ hybridization (ISH) procedure for HPV. In situ hybridization detection system from Dako/UK was used to detect HPV 16&18 according to the manufactures instructions. Current results found the positive HPV in cervical lesions represented (79.4%) with significant differences (**P<0.01), while the negative HPV cervical lesions represented (20.6%). Age group (34-44) was the highest frequency and percentage 17 (63%) of patients positive cervical lesions. the histopathology and cytology diagnosis showed that a high percentage in the patients under our study have positive HPV in cervical lesions represented (79.4%).In addition positive nuclear cells expression of HPV in two type of samples (cytology and Histopathology) were examined, histopathological samples ( 11/15, 73.3% ) and cytology samples (12/15,80%) were positive to HPV in cervical lesions . while 4/4, 100% was positive HPV for both sample types. Expression pattern was highly significant as punctated and diffused (48.2%) compared to Punctate expression(7.4%). In conclusion, HPV detection in cytological specimens and histological by ISH molecular method were help to distinguish integrated HPV- DNA form, thus allowing conclusions about the prognosis of the cervical lesions abnormalities.
Keywords: Human papilloma virus; Cervix; In situ hybridization
References
Bonnez, W. and Reichman, R.C. Principles and Practice of Infectious Diseases. Churchill Livingstone, Philadelphia, 2000, pp. 1630-1644.
Zappa costa, R. and Rosini, S. “Cervical cancer screening: from molecular basis to diagnostic practice, going through new technologies,” Technology in Cancer Research and Treatment, 2008 , 7( 3), : 161–174.
Cuzick, C. Clavel, K. Petry et al., “Overview of the European and North American studies on HPV testing in primary cervical cancer screening,” International Journal of Cancer. 2006,119(5): 1095–1101.
Cuzick, M. ; Arbyn, R. ;Sankaranarayanan et al., “Overview of human papillomavirus-based and other novel options for cervical cancer screening in developed and developing countries.Vaccine. 2008, 26(10): K29–K41.
Yoshinouchi, M.; Hongo, A. ; Nakamura, K.; Kodoma, J.; Itoh, S. et al. Journal of Clinical Microbiology. 1999,37:3514 .
Syrjänen, S.M. andSyrjänen, K.J. Annals of Medicine. 1999, 31: 175.
Evans, M. F. ; Mount, S. L. ; Beatty, B. G. and Cooper, K. Biotinyltyramide - based in situ hybridization signal patterns distinguish human papillomavirus type and grade of cervical ntraepithelial neoplasia. Modern Pathology . 2002, 15( 12) : 1339–1347.
Evans, M. F. and Cooper, K. Human papillomavirus integration: detection by in situ hybridization and potential clinical application. Journal of Pathology. 2004, 202( 1,): 1–4.
Montag, M . ;Blankenstein, TJ.; Shabani, N. ;Brüning, A.; Mylonas, I. Evaluation of two commercialised in situ hybridisation assays for detecting HPV-DNA in formalinfixed,paraffin-embedded tissue. Archives of Gynecology and Obstetrics. 2011 ,284( 4) : 999–1005.
Zappacosta, R.; Colasante,A. ;Viola,P. and D’Antuono,T. et al. In Situ Hybridization and p16/Ki67Dual Staining on Formalin-Fixed Paraffin-Embedded Cervical Specimens: Correlation with HPV-DNA Test, E6/E7 mRNA Test,and Potential Clinical Applications. BioMed Research International . 2013,Article ID 453606, 11 pages, 1-11 pages.
SAS. Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA, 2012.
Gloria, B.; Rolda´n, U.; Karla. G.; Alexander C. et al., The Prognostic Value of HPV Status and p16 Expressionin Patients with Carcinoma of the Anal Canal .PLoS ONE. 2014, 9(10): e108790.
Mark F. Evans , Zhihua Peng, Kelli M. Clark, Christine S. and et al. HPV E6/E7 RNA In Situ Hybridization Signal Patterns as Biomarkers of Three-Tier Cervical Intraepithelial Neoplasia Grade. PLoS ONE .2014;9(3): e91142.
Theodoros, K.; Leo ,A.; Michael, A. S. et al. Human Papillomavirus (HPV) Detection Using In SituHybridization in Histologic Samples Correlations With Cytologic Changes and Polymerase Chain Reaction HPV Detection. Am J Clin Pathol. 2011;136 :119-127.
Murphy, N.; Ring, M. ; Heffron , B. et al., p16INK4a, CDC6,and MCM5: predictive biomarkers in cervical preinvasive neoplasia and cervical cancer . Journal of Clinical Pathology. 2005, 58( 5): 525–534.
Wang, S. S.; Trunk, M; Schiffman , M.; et al., Validation of p16 INK4a as a marker of oncogenic human papillomavirus infection in cervical biopsies from a population-based cohort inCosta Rica. Cancer Epidemiology Biomarkers and Prevention. 2004, 13( 8) : 1355–1360.
READ THE FULL ARTICLE
1. Access this article through OpenAthens
2. Access this article through your login credentials or your institution
3. Purchase this article at rate $55.00 and received Full-Text/PDF
You will have online immediate access to article following the completion of this purchase and you may download and print a copy of each article for your personal use. Use the coding below to purchase your article as PDF by credit card, debit card, will be asked to supply your billing card information. Before continue with your purchase please read carefully BM–Publisher terms and conditions of purchase.
For any technique error please contact us and will be response to sending purchase article by email.
Thank you for visiting Journal of Cellular Cancer. * = Required fields
